Culture and Development

Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

The Impact of Components of Organizational Culture on Components of the Faculty Members᾽ Development

Introduction: Organizational culture is considered as an important element in the body of an organization that has a positive impact on the performance of the every organization, so that the success of any organization depends on culture of the organization, partly. Much research has been done on the role of this factor in promoting organizational productivity and achieving organizational goals...

O-16: Comparison of Pre-Antral Follicle Culture Development during 2 Dimensional and 3 Dimensional Culture Systems

Background: Setting up an in vitro follicle culture system that resembles in vivo ovary condition has high value in research. Additionally, expression evaluation of folliculogenesis involved genes could lead us to the designing of better culture system. Materials and Methods: ovaries of 12-day-old female NMRI mice were removed, 100-130 μm pre-antral follicles were mechanically isolated from fre...

A Sociological Study of the Culture of Development in Hawrami Proverbs

The present study investigates the culture of development in Hawrami proverbs. Analysis of development-related variables is the basis of sociology. As collective phenomena, proverbs are part of the oral culture of a society and their investigation can sociologically explain cultural and social foundations of the society. This research attempts to specify 6 development-related parameters in Hawr...

Steroid Production and Follicular Development of Neonatal Mouse Ovary during In Vitro Culture

Effect of LH Treated Ovine Oviductal Epithelial Cell Co-Culture System on Murine Pre-Embryo Development

Background This study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (LH) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. MaterialsAndMethods Monolayers for co-culture were prepared from ovine oviduct epithelial cells (OOEC) in DMEM/F12 medium a...